More details for each step are in the Owner's Manual, page numbers are referenced below.

 

A mix-minus allows you to create an alternate audio mix where one or more sources are removed from the main mix.

 

This is done by setting a video output's audio bus assignment to AUX and then muting the AUX Send for any audio channel you want to remove from the AUX audio mix.

 

In this example, we set up the USB Streaming Output for a mix-minus.

 

First, go to Menu > USB Streaming and set the Audio Bus to AUX.

(Owner's Manual, page 21)

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Next, press the Setup button above the Main fader and set the AUX Level to 0.0 dB.

(Owner's Manual, page 30)

Note: If you hold the Enter button while turning the Value knob, it'll change the setting faster.

 

You should also determine on this screen which audio mix is assigned each video output, if needed.

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Finally, press the Setup button above each audio input fader and set the AUX Send level to -INF if you want to remove it from the AUX audio mix or 0.0dB if you want it to add it to the AUX audio mix.

(Owner's Manual, page 29)

 

The AUX Send value is post-fader, which means that the faders you use to adjust the Main audio mix will also affect the AUX audio mix.

So, if you turn Mic 1 all the way down and mute it, it will be muted in the AUX Mix as well.

Note: The AUX Send value therefore either lowers, passes-through, or raises the level it receives from the audio channel's fader. In most cases, you will set the AUX Send value to either -INF or 0.0dB.​